AccuLNP is designed to meet the diverse needs of researchers across various fields. Whether you’re developing novel mRNA therapies, advancing gene editing, or conducting fundamental research, AccuLNP provides the flexibility and reliability necessary for success.
A total of 150 ng of GFP mRNA, encapsulated using our AccuLNP Transfection Reagent, was delivered into HEK293, Hep3B, and MCF7 cells cultured in a 96-well plate. At 24 hours post-transfection, cells were stained with DAPI and visualized under a fluorescence microscope. The results demonstrate that the AccuLNP Transfection Reagent is highly effective across a diverse range of cell types.
HEK293 cells in a 96-well plate were transfected with 150 ng of GFP mRNA encapsulated using either our AccuLNP Transfection Reagent or a leading competitor’s transfection kit. At 24 hours post-transfection, cells were stained with DAPI and examined using fluorescence microscopy. The results clearly show that the AccuLNP Transfection Reagent outperforms competing transfection reagents in efficiency and effectiveness.
In addition to GFP mRNA, AccuLNP also demonstrates exceptional transfection efficiency with firefly luciferase (FLuc) mRNA in vitro. In this experiment, 500 ng of FLuc mRNA was encapsulated using the AccuLNP Transfection Reagent and transfected into HEK293 cells cultured in a 24-well plate. The cells were assessed at 24 hours post-transfection for luciferase expression using a luminescence assay, with results normalized to the competitor’s transfection efficiency.
The results clearly show that AccuLNP delivers superior efficiency in FLuc mRNA transfection, highlighting its versatility and performance across different mRNA applications.
AccuLNP is available in two formulations: one optimized for in vitro use and another specifically designed for in vivo applications. The following data show the results of an in vivo experiment using the AccuLNP in vivo version to deliver firefly luciferase (FLuc) mRNA.
For this experiment, 0.2 mg/kg of FLuc mRNA was administered via intravenous injection using the in vivo version of the AccuLNP Transfection Reagent. Luciferase expression was measured at a single time point following injection to assess transfection efficiency.